Application of 1953-54-4, The reaction rate of a catalyzed reaction is faster than the reaction rate of the uncatalyzed reaction at the same temperature.1953-54-4, Name is 5-Hydroxyindole, molecular formula is C8H7NO. In a Article£¬once mentioned of 1953-54-4
Sulfation of various alcoholic groups by an arylsulfate sulfotransferase from desulfitobacterium hafniense and synthesis of estradiol sulfate
Bacterial arylsulfate sulfotransferases (AST) are enzymes that catalyse the transfer of a sulfate group from p-nitrophenyl sulfate (p-NPS) to a phenolic acceptor molecule. By screening of the NCBI protein database a gene coding for an AST was found in Desulfitobacterium hafniense. After expression the enzyme was purified and characterised. This AST efficiently sulfates various acceptor molecules (estrone, estradiol, enkephalin and non-phenolic alcohols) using p-NPS as sulfate donor. The purified AST has a pH optimum of 9.6, it is stable in the presence of 10% of DMSO, and depending on the conditions it has a melting temperature of up to 47C. Surprisingly, and in great contrast to all other known bacterial ASTs, this enzyme was able to use a variety of non-phenolic alcohols as sulfate acceptor. Because of these properties, this unique enzyme is a promising tool for biotransformation processes, providing a green and simple method to specifically sulfate compounds without need for functional group protection.
A reaction mechanism is the microscopic path by which reactants are transformed into products. Each step is an elementary reaction. In my other articles, you can also check out more blogs about 1953-54-4
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Indole alkaloid derivatives as building blocks of natural products from?Bacillus thuringiensis?and?Bacillus velezensis?and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles