Reactions catalyzed within inorganic and organic materials and at electrochemical interfaces commonly occur at high coverage and in condensed media, causing turnover rates to depend strongly on interfacial structure and composition, 533-73-3, Name is Benzene-1,2,4-triol, SMILES is OC1=CC=C(O)C=C1O, in an article , author is Manokari, M., once mentioned of 533-73-3, Computed Properties of C6H6O3.
Direct somatic embryogenesis using leaf explants and short term storage of synseeds in Spathoglottis plicata Blume
Spathoglottis plicata Blume is a vulnerable orchid species in various parts of the world, and the conventional propagation provides limited success to its cultivation and conservation. Therefore, present study deals with the direct induction of somatic embryos (SEs) from the leaf explants of S. plicata. Murashige and Skoog’s (MS) medium fortified with various types and concentrations of plant growth regulators were used to induce somatic embryogenesis and plantlet production. The highest percentage of somatic embryo formation (93.7 +/- 0.56%) was achieved on MS medium supplemented with 1.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), whereas, maximum proliferation and increase in fresh weight (FW) of SEs (149.5 +/- 0.24 mg/ 50 mg initial FW) was achieved on MS medium fortified with 2.0 mg L-1 6-benzylaminopurine (BAP) within 5 weeks of incubation. The light microscopic observation revealed that SEs emerged directly from the leaf surface. The viable synthetic seeds (SS) from SEs were prepared by encapsulating with gel matrix of 3% (w/v) sodium alginate and 100 mM calcium chloride. The SS were successfully stored for 60 days at – 4 degrees C with 97.4% germination frequency and shoot proliferation using MS medium with 2.0 mg L-1 BAP and 0.25 mg L-1 indole-3-acetic acid. The synergistic rooting frequency was observed on half-strength MS medium with 1.0 mg L-1 indole-3-butyric acid. The rooted shoots were acclimatized in a greenhouse with a 90% survival rate using soilrite (R) and vermicompost. This is an efficient short-term storage and regeneration protocol for S. plicata, which could help in reducing pressure on its wild population and could also be extended to the cryopreservation of this orchid species.
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Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles