An article Growth Kinetics, Metabolites Production and Expression Profiling of Picrosides Biosynthetic Pathway Genes in Friable Callus Culture ofPicrorhiza kurroaRoyle ex Benth WOS:000553276000002 published article about PLANT-PICRORHIZA-KURROA; ANTIOXIDANT ACTIVITY; MEDICINAL HERB; SECONDARY METABOLITES; CONSERVATION; REGENERATION; ENHANCEMENT in [Partap, Mahinder; Kumar, Pankaj; Ashrita; Warghat, Ashish R.] CSIR, Biotechnol Div, Cell & Tissue Engn Lab, Inst Himalayan Bioresource Technol, Palampur 176061, Himachal Prades, India; [Partap, Mahinder; Ashrita; Kumar, Dinesh; Warghat, Ashish R.] Acad Sci & Innovat Res, New Delhi, India; [Kumar, Pawan; Kumar, Dinesh] CSIR, Nat Prod Chem & Proc Dev Div, Inst Himalayan Bioresource Technol, Palampur 176061, Himachal Prades, India in 2020.0, Cited 41.0. Product Details of 99-93-4. The Name is 4′-Hydroxyacetophenone. Through research, I have a further understanding and discovery of 99-93-4
The rising demand for picrosides commercially and over-exploitation ofPicrorhiza kurroafrom natural habitat has to initiate alternative strategies for sustainable production of metabolites. In the present research, wild leaf explant ofP. kurroawas used to produce friable callus under different culture condition, i.e., dark and light with two temperature variants (15 degrees C and 25 degrees C). Afterward, callus cell lines were screened based on growth biomass and metabolites content accumulation. The results revealed, maximum callus growth index along with antioxidant potential (IC50-40.88 mu g/mL) and total phenol content (41.35 mu g/mg) were observed under dark 25 degrees C. However, under light 15 degrees C, highest accumulation of picroside II (0.58 mu g/mg), cinnamic acid (0.15 mu g/mg),p-hydroxy acetophenone (0.30 mu g/mg), total flavonoids (77.30 mu g/mg), nitrogen (7.06%), carbohydrates (18.03%), and protein (44.12%) were detected. Major reported metabolite in callus was picroside I (1.63 mu g/mg) under dark 15 degrees C. For the first time, picroside III content (range 0.15-0.56 mu g/mg) was also detected and quantified in leaf-derived calli. Expression profiling of picroside biosynthetic pathway genes showed a positive correlation with the observed metabolites. Furthermore, an optimized protocol of metabolites enriched callus biomass could be used as potential strategy for sustainable production of picrosides at commercial scale.
Product Details of 99-93-4. About 4′-Hydroxyacetophenone, If you have any questions, you can contact Partap, M; Kumar, P; Ashrita; Kumar, P; Kumar, D; Warghat, AR or concate me.
Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles