Chemo-enzymatic cascade processes are invaluable due to their ability to rapidly construct high-value products from available feedstock chemicals in a one-pot relay manner. In an article, author is Kyriienko, A., V, once mentioned the application of 6915-15-7, Name is DL-Malic acid, molecular formula is C4H6O5, molecular weight is 134.0874, MDL number is MFCD00064212, category is indole-building-block. Now introduce a scientific discovery about this category, Recommanded Product: DL-Malic acid.
The present study reports an efficient in vitro plant regeneration system for amphidiploid (2n = 42) spelt wheat (Triticum spelta L.). Plant regeneration from mature embryos of winter spelt variety Europe was carried out as a two-step process. The first step was callus induction on a medium supplemented with 2 mg L-1 dichlorophenoxyacetic acid and 10 mg L-1 silver nitrate resulting in 96% callus formation. The second step resulted in 30% plant regeneration frequency from calluses transferred to Murashige and Skoog medium supplemented with 2 mg L-1 6-benzylaminopurine, 0.5 mg L-1 1-naphthaleneacetic acid, and 10 mg L-1 silver nitrate. Regeneration medium supplemented with 1 mg L-1 6-benzylaminopurine, 0.2 mg L-1 picloram, and the hormone-free medium turned out to be less effective in our experiments. Regenerated plants formed roots after transfer to half-strength Murashige and Skoog medium supplemented with 0.7 mg L-1 indole-3-butyric acid. About one-third of the resulting regenerated plants that formed roots were transferred to soil. The inter-simple sequence repeat markers were used to confirm the genetic homogeneity of regenerated plants. Thus, our regeneration protocol can be used for in vitro regeneration of spelt plants from mature embryos with minimal risk of genetic variability and provides an essential step towards developing an efficient strategy for spelt genetic transformation.
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Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles