Authors Srividya, N; Lange, I; Hartmann, M; Li, QR; Mirzaei, M; Lange, BM in AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC published article about COENZYME-A-SYNTHETASE; COA SYNTHETASE; ESCHERICHIA-COLI; ARABIDOPSIS-THALIANA; ANALYSIS REVEALS; BIOSYNTHESIS; ACID; IDENTIFICATION; LIGASES; GROWTH in [Lange, Bernd Markus] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA; Washington State Univ, MJ Murdock Metabol Lab, Pullman, WA 99164 USA; [Hartmann, Michael] Univ Dusseldorf, Dept Mol Ecophysiol Plants, Univ Str 1, D-40225 Dusseldorf, Germany; [Li Qunrui] CP Pharmaceut Grp, Jia 3 Yongandongli, Beijing 100022, Peoples R China; [Mirzaei, Maryam] Tarbiat Modares Univ, Tehran 14115111, Iran in 2020, Cited 41. HPLC of Formula: C5H8O2. The Name is (E)-2-Methylbut-2-enoic acid. Through research, I have a further understanding and discovery of 80-59-1
Taxol (paclitaxel) is a very widely used anticancer drug, but its commercial sources mainly consist of stripped bark or suspension cultures of members of the plant genus Taxus. Taxol accumulates as part of a complex mixture of chemical analogs, termed taxoids, which complicates its production in pure form, highlighting the need for metabolic engineering approaches for high-level Taxol production in cell cultures or microbial hosts. Here, we report on the characterization of acyl-activating enzymes (AAEs) that catalyze the formation of CoA esters of different organic acids relevant for the N-substitution of the 3-phenylisoserine side chain of taxoids. On the basis of similarities to AAE genes of known function from other organisms, we identified candidate genes in publicly available transcriptome data sets obtained with Taxus ? media. We cloned 17 AAE genes, expressed them heterologously in Escherichia coli, purified the corresponding recombinant enzymes, and performed in vitro assays with 27 organic acids as potential substrates. We identified TmAAE1 and TmAAE5 as the most efficient enzymes for the activation of butyric acid (Taxol D side chain), TmAAE13 as the best candidate for generating a CoA ester of tiglic acid (Taxol B side chain), TmAAE3 and TmAAE13 as suitable for the activation of 4-methylbutyric acid (N-debenzoyl-N-(2-methylbutyryl)taxol side chain), TmAAE15 as a highly efficient candidate for hexanoic acid activation (Taxol C side chain), and TmAAE4 as suitable candidate for esterification of benzoic acid with CoA (Taxol side chain). This study lays important groundwork for metabolic engineering efforts aimed at improving Taxol production in cell cultures.
HPLC of Formula: C5H8O2. About (E)-2-Methylbut-2-enoic acid, If you have any questions, you can contact Srividya, N; Lange, I; Hartmann, M; Li, QR; Mirzaei, M; Lange, BM or concate me.
Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles