Simultaneous determination of tryptophan and its 31 catabolites in mouse tissues by polarity switching UHPLC-SRM-MS was written by Chen, Guan-yuan;Zhong, Wei;Zhou, Zhanxiang;Zhang, Qibin. And the article was included in Analytica Chimica Acta in 2018.Safety of Indole-3-acetamide This article mentions the following:
Tryptophan (TRP) and its catabolites have attracted a lot of attention because of their clin. significance to human health. Recently, microbiome-gut-brain axis has links to many diseases based on the imbalance of TRP catabolism. By using ultra-HPLC coupled to electrospray ionization triple quadrupole mass spectrometry, the authors present a rapid, robust and comprehensive method to determine 31 TRP catabolites covering three major pathways – kynurenic, serotonergic and bacterial degradation – within 5 min. Polarity switching was employed to analyze catabolites in both ionization modes simultaneously for greatly improved anal. throughput. The intra-day and inter-day precision were 0.5-15.8% and 1.5-16.7%, resp. Accuracy was 75.8-126.9%. The developed method was applied to study the tissue level of TRP catabolites in the liver, ileum, ileal contents, brain and plasma samples from 8 mice, and clear differences in the distribution of TRP catabolites were observed in different tissues. Ratios of key catabolites to TRP were used to evaluate the activities of specific enzyme and pathway in resp. tissues. This method has potential in high throughput anal. of TRP catabolites in biol. matrixes, which can facilitate understanding the influence of TRP catabolites on microbiome-gut-brain axis and on human health. In the experiment, the researchers used many compounds, for example, Indole-3-acetamide (cas: 879-37-8Safety of Indole-3-acetamide).
Indole-3-acetamide (cas: 879-37-8) belongs to indole derivatives. Indole exists overwhelmingly in the 1H-indole form as do other simple indoles. Moreover, it is known that it controls biofilm formation. However, the role of indole in the cell has not been fully elucidated.Safety of Indole-3-acetamide
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles