Metabolomics coupled with network pharmacology study on the protective effect of Keguan-1 granules in LPS-induced acute lung injury was written by Chen, Shuaishuai;Zhou, Mingxi;Zhao, Xu;Han, Yanzhong;Huang, Ying;Zhang, Long;Wang, Jiabo;Xiao, Xiaohe;Li, Pengyan. And the article was included in Pharmaceutical Biology (Abingdon, United Kingdom) in 2022.Computed Properties of C10H10N2O This article mentions the following:
Keguan-1 (KG-1) plays a vital role in enhancing the curative effects, improving quality of life, and reducing the development of acute lung injury (ALI). To unravel the protective effect and underlying mechanism of KG-1 against ALI. C57BL/6J mice were intratracheally instilled with lipopolysaccharide to establish the ALI model. Then, mice in the KG-1 group received a dose of 5.04 g/kg for 12 h. The levels of proinflammatory cytokines, chemokines, and pathol. characteristics were determined to explore the effects of KG-1. Next, untargeted metabolomics was used to identify the differential metabolites and involved pathways for KG-1 anti-ALI. Network pharmacol. was carried out to predict the putative active components and drug targets of KG-1 anti-ALI. KG-1 significantly improved the levels of TNF-伪 (from 2295.92 卤 529.87 pg/mL to 1167.64 卤 318.91 pg/mL), IL-6 (from 4688.80 卤 481.68 pg/mL to 3604.43 卤 382.00 pg/mL), CXCL1 (from 4361.76 卤 505.73 pg/mL to 2981.04 卤 526.18 pg/mL), CXCL2 (from 5034.09 卤 809.28 pg/mL to 2980.30 卤 747.63 pg/mL), and impaired lung histol. damage. Untargeted metabolomics revealed that KG-1 significantly regulated 12 different metabolites, which mainly related to lipid, amino acid, and vitamin metabolism Network pharmacol. showed that KG-1 exhibited anti-ALI effects through 17 potentially active components acting on seven putative drug targets to regulate four metabolites. Discussion and conclusionsThis work elucidated the therapeutic effect and underlying mechanism by which KG-1 protects against ALI from the view of the metabolome, thus providing a scientific basis for the usage of KG-1. In the experiment, the researchers used many compounds, for example, Indole-3-acetamide (cas: 879-37-8Computed Properties of C10H10N2O).
Indole-3-acetamide (cas: 879-37-8) belongs to indole derivatives. Indole is an important structural motif of various drugs, therapeutic leads besides its prevalence in numerous natural products, agrochemicals, perfumery, and dyes.Indole was synthesized in moderate yield via an o-naphthoquinone catalyzed tandem cross-coupling of substituted aniline and benzylamine under aerobic oxidation conditions.Computed Properties of C10H10N2O
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Indole alkaloid derivatives as building blocks of natural products from聽Bacillus thuringiensis聽and聽Bacillus velezensis聽and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles