On September 20, 2017, Dressen, Alana; Hilberath, Thomas; Mackfeld, Ursula; Rudat, Jens; Pohl, Martina published an article.COA of Formula: C9H9NO2 The title of the article was Phenylalanine ammonia lyase from Arabidopsis thaliana (AtPAL2): A potent MIO-enzyme for the synthesis of non-canonical aromatic alpha-amino acids.. And the article contained the following:
Phenylalanine ammonia lyase (PAL) from Arabidopsis thaliana (AtPAL2) is in general a very good catalyst for the amination of fluoro- and chloro-cinnamic acid derivatives yielding halogenated (S)-phenylalanine derivatives with ≥85% conversion and excellent ee values >99%. We have studied the application of this enzyme as whole cell biocatalyst and immobilized on the cellulose carrier Avicel for the production of the hypertension drug precursor (S)-2-chloro-phenylalanine using batch, fed-batch, as well as continuous membrane reactor and plug-flow reactor. For immobilization, a C-terminal fusion of the enzyme with a carbohydrate binding module (CBM) was produced, which selectively binds to Avicel directly from crude cell extracts, thus enabling a fast and cheap immobilization, stabilization and recycling of the enzyme. 1 g Avicel was loaded with 10 mg enzyme. Best results were obtained with whole cells using the continuous membrane reactor (47 gproduct/gDryCellWeight) and using the immobilized enzyme in a repetitive fed-batch (274 gproduct/gimmobilized enzyme) or in a continuous plug-flow reactor (288 gproduct/gimmobilize enzyme). Therewith the productivity of AtPAL2 outperforms the established fed-batch process at DSM using PAL from Rhodotorula glutinis in E. coli as whole cell biocatalyst with a productivity of 0.14 gproduct/gWetCellWeight (0.7 gproduct/gDryCellWeight) (de Lange et al., 2011; doi:10.1002/cctc.201000435). The experimental process involved the reaction of H-Idc-OH(cas: 79815-20-6).COA of Formula: C9H9NO2
The Article related to phenylalanine ammonia lyase arabidopsis noncanonical aromatic amino acid, (s)-2-cl-phe (pubchem cid85679), (s)-2-indolinecarboxylic acid, (s)-phe (pubchem cid: 6140), 2-cl-cinnamic acid (pubchem cid: 700642), enzyme reactor, indolapril, mio enzyme, perindopril, trans-cinnamic acid (pubchem cid: 444539) and other aspects.COA of Formula: C9H9NO2
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Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles