Formula: C7H8O2. Authors Kwon, J; Kang, HY; Yang, H in ELSEVIER SCIENCE SA published article about in [Kwon, Jungwook; Yang, Haesik] Pusan Natl Univ, Dept Chem, Busan 46241, South Korea; [Kwon, Jungwook; Yang, Haesik] Pusan Natl Univ, Chem Inst Funct Mat, Busan 46241, South Korea; [Kang, Ho Young] Pusan Natl Univ, Dept Microbiol, Busan 46241, South Korea in 2021.0, Cited 25.0. The Name is Mequinol. Through research, I have a further understanding and discovery of 150-76-5
Escherichia coli (E. coli) is recognized as an indicator of fecal bacterial contamination, and the induction of intracellular beta-galactosidase (Gal) is commonly used for sensitive and selective E. coli detection. However, this method requires a permeabilization process to ensure that the Gal substrate and product are highly permeable through the E. coli membrane, making it unsuitable for simple point-of-care detection. Here, we report a sensitive electrochemical method for E. coli detection that does not require a permeabilization process. Intracellular Gal expression is increased via induction by isopropyl-beta-D-thiogalactopyranoside (IPTG). 4-Methoxyphenyl-beta-D-galactopyranoside (MPGP) and 4-methoxyphenol (MP) are used as the highly cell-permeable Gal substrate and product, respectively. Externally-added tyrosinase (Tyr) converts MP into electrochemically active 4-methoxycatechol, which is then electrochemically oxidized via electrochemical-chemical redox cycling involving an electrode, 4-methoxycatechol, and tris(2-carboxyethyl)phosphine. The presence of MPGP during the induction of intracellular Gal led to amplified MP production. The results of four different detection methods using three Gal substrates (MPGP, phenyl-beta-D-galactopyranoside, and 4-aminophenyl-beta-D-galactopyranoside) were compared. The detection limit for both drinking water and tap water spiked with E. coli, obtained following a 2-h-long IPTG treatment and 5-min-long Tyr reaction, was similar to 2 x 10(3) colony-forming units (CFU)/mL, indicating that this method can be applied to monitoring E. coli contamination in water samples. Importantly, the present method does not require the electrode modification, affinity binding, washing, and filtration steps.
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Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles