Kajihara, Yasuhiro; Kodama, Hisashi; Endo, Tsuyoshi; Hashimoto, Hironobu published an article on January 31 ,1998. The article was titled 《Novel features of acceptor recognition by β-(1→4)-galactosyltransferase》, and you may find the article in Carbohydrate Research.Formula: C22H25NO9S The information in the text is summarized as follows:
In order to understand how β-(1→4)-galactosyltransferase recognizes its glycosyl acceptor, substrate specificities were investigated using synthetic 2-acetamido-2-deoxy-D-glucopyranose (N-acetylglucosamine) derivatives in which the 1-, 2-, 3-, 4-, and 6-positions were systematically substituted. The hydroxyl groups at the 3-, 4-, and 6-positions were substituted by fluoride, thiol or hydrogen. For modification of the 2- position, the acetamido group was converted to ethylamino-, N-methylacetamido- and acetyloxy groups. For the anomeric position, several sugar residues were introduced as the aglycon of N-acetylglucosaminide. Galactose transfer assay using synthetic N-acetylglucosamine derivatives indicated that both the acetamido group and the 4-hydroxyl group were essential for binding of N-acetylglucosamine toward the β-(1→4)-galactosyltransferase. The assay also showed that the N-acetylglucosamine having a large substitution at the 6-position can be recognized as an acceptor. It is suggested that in this case the bulky substituent is positioned away from the catalytic site or out of enzyme. Since the 2-acetamido and the 4-hydroxyl group are essential for recognition, the side composed of the 2, 3, and 4-positions may face the acceptor-binding site. The experimental process involved the reaction of Ethyl 3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-β-D-thioglucopyranoside(cas: 99409-32-2Formula: C22H25NO9S)
Ethyl 3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-β-D-thioglucopyranoside(cas: 99409-32-2) belongs to indole.The indole subunit is an almost ubiquitous component of biologically active natural products, and its study has been the focus of research for decades.Formula: C22H25NO9SThey are capable of binding to a variety of receptors with high affinity and thus have applications in a wide range of therapeutic areas.
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles