Reversed phase planar chromatography of enantiomeric tryptophans with bovine serum albumin in the mobile phase was written by Lepri, Luciano;Coas, Vanda;Desideri, Pier Giorgio;Zocchi, Andrea. And the article was included in Journal of Planar Chromatography–Modern TLC in 1992.Formula: C12H14N2O2 This article mentions the following:
The chromatog. behavior of racemic tryptophan, tryptophanamide, glycyltryptophan, and eight substituted tryptophans on several types of reversed phase layers developed with aqueous organic solutions containing high amounts of bovine serum albumin (BSA) has been extensively investigated. The resolution of the enantiomers is highly dependent on the apparent pH of the eluent; in particular, the best conditions for their resolution have been obtained in the pH range 9-10. The amount of 2-propanol in the mobile phase also plays an important role, as does the type of layer; some of the octadecyl layers, for example, could not be used with an eluent pH higher than 9.55. The α and Rs values of the different enantiomers are generally higher than those achieved on microcrystalline cellulose. BSA in the mobile phase can be successfully employed for the separation of a large number of racemic compounds In the experiment, the researchers used many compounds, for example, 2-Amino-3-(7-methyl-1H-indol-3-yl)propanoic acid (cas: 17332-70-6Formula: C12H14N2O2).
2-Amino-3-(7-methyl-1H-indol-3-yl)propanoic acid (cas: 17332-70-6) belongs to indole derivatives. In addition to tryptophan, indigo, and indoleacetic acid, numerous compounds obtainable from plant or animal sources contain the indole molecular structure. Indole plays a fundamental role for QS in E. coli, being one of the signal molecules responsible for the transcription of a variety of genes (gabT, and tnaB ASTD). Formula: C12H14N2O2
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles