Efficient synthesis of novel spiro[indoline-3,5′-pyrano-[2,3-d]pyrimidin]-2-one derivatives and antitumor activity evaluation was written by Li, Zhenhua;Huang, Guoqing;Rong, Dayou;Cao, Yingyan;Hu, Ronghui. And the article was included in Heterocycles in 2022.Synthetic Route of C8H4N2O4 This article mentions the following:
An efficient method for synthesis of the spiro[indoline-3,5′-pyrano[2,3-d]pyrimidin]-2-one derivatives I [R1 = H, 7-Me, 5-Cl, etc.; R2 = H, Me; R3 = CN, C(O)Me, CO2Et; R4 = Me, Ph] from 2′-amino-2-oxospiro[indoline-3,4;-pyran]-3′-carbonitriles using bis(trichloromethyl) carbonate (BTC) and triphenylphosphine oxide (TPPO) was developed. A series of target compounds I with broad substrate scope were synthesized in moderate to good yields. Some of the compounds I were evaluated for antitumor activities against four cancer cell lines A549, HepG-2, MCF-7 and HeLa using 5-FU and cisplatin as reference and showed good antitumor activity when compared with the standard drugs. In the experiment, the researchers used many compounds, for example, 7-Nitroindoline-2,3-dione (cas: 112656-95-8Synthetic Route of C8H4N2O4).
7-Nitroindoline-2,3-dione (cas: 112656-95-8) belongs to indole derivatives. In addition to tryptophan, indigo, and indoleacetic acid, numerous compounds obtainable from plant or animal sources contain the indole molecular structure. They are capable of binding to a variety of receptors with high affinity and thus have applications in a wide range of therapeutic areas.Synthetic Route of C8H4N2O4
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles