miR-1 is increased in pulmonary hypertension and downregulates Kv1.5 channels in rat pulmonary arteries was written by Mondejar-Parreno, Gema;Callejo, Maria;Barreira, Bianca;Morales-Cano, Daniel;Esquivel-Ruiz, Sergio;Moreno, Laura;Cogolludo, Angel;Perez-Vizcaino, Francisco. And the article was included in Journal of Physiology (Oxford, United Kingdom) in 2019.Product Details of 194413-58-6 This article mentions the following:
Key points : The expression of miR-1 is increased in lungs from the Hyp/Su5416 PAH rat model. Pulmonary artery smooth muscle cells from this animal model are more depolarized and show decreased expression and activity of voltage-dependent potassium channel (Kv)1.5. miR-1 directly targets Kv1.5 channels, reduces Kv1.5 activity and induces membrane depolarization. Antagomir-1 prevents Kv1.5 channel downregulation and the depolarization induced by hypoxia/Su5416 exposition. Impairment of the voltage-dependent potassium channel (Kv) plays a central role in the development of cardiovascular diseases, including pulmonary arterial hypertension (PAH). MicroRNAs are non-coding RNAs that regulate gene expression by binding to the 3′-untranslated region region of specific mRNAs. The present study aimed to analyze the effects of miR-1 on Kv channel function in pulmonary arteries (PA). Kv channel activity was studied in PA from healthy animals transfected with miR-1 or scrambled-miR. Kv currents were studied using the whole-cell configuration of the patch clamp technique. The characterization of the Kv1.5 currents was performed with the selective inhibitor DPO-1. MiR-1 expression was increased and Kv1.5 channels were decreased in lungs from a rat model of PAH induced by hypoxia and Su5416. MiR-1 transfection increased cell capacitance, reduced Kv1.5 currents and induced membrane depolarization in isolated pulmonary artery smooth muscle cells. A luciferase reporter assay indicated that KCNA5, which encodes Kv1.5 channels, is a direct target gene of miR-1. Incubation of PA with Su5416 and hypoxia (3% O2) increased miR-1 and induced a decline in Kv1.5 currents, which was prevented by antagomiR-1. In conclusion, these data indicate that miR-1 induces pulmonary artery smooth muscle cell hypertrophy and reduces the activity and expression of Kv channels, suggesting a pathophysiol. role in PAH. In the experiment, the researchers used many compounds, for example, (Z)-3-((3,5-Dimethyl-1H-pyrrol-2-yl)methylene)indolin-2-one (cas: 194413-58-6Product Details of 194413-58-6).
(Z)-3-((3,5-Dimethyl-1H-pyrrol-2-yl)methylene)indolin-2-one (cas: 194413-58-6) belongs to indole derivatives. Indole could be stereoselectively alkylated with chiral cyclopentyl sulfone reagent. Due to this activity, the indole ring system has become an important component or intermediate in the synthesis of heterocycles.Product Details of 194413-58-6
Referemce:
Indole alkaloid derivatives as building blocks of natural products from聽Bacillus thuringiensis聽and聽Bacillus velezensis聽and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles