Chemistry is an experimental science, Quality Control of Aloe-emodine, and the best way to enjoy it and learn about it is performing experiments.Introducing a new discovery about 481-72-1, Name is Aloe-emodine, molecular formula is C15H10O5, belongs to indole-building-block compound. In a document, author is Han Runchuan.
Determination of indoles in plasma of polycystic ovarian syndrome patients by high performance liquid chromatography-fluorescence detection
A new method was established for the determination of indoles (indole (IND), 3methylindole (3 -MI), indolyl-3-acetic acid (IAA) and indolyl-3-propionic acid (IPA)) in plasma by high performance liquid chromatography-fluorescence detection (HPLC-FLD). The analytes were separated simultaneously on a Shim -pack VP-ODS column (150 mmx4. 6 mm, 4. 6 tim) using 15 mmol/L sodium dihydrogen phosphate solution and methanol (48 :52, v/v) as the mobile phases. The column temperature was 30 C, and the flow rate was 0. 8 mL/min. The calibration curves of IND, 3 -MI, IPA and IAA showed good linear relationships. The intra-day and inter-day relative standard deviations (RSDs) for the analytes were both less than 6. 31%. The average recoveries of the analytes in plasma were 97. 5% 107. 0%. The method was successfully applied to the analysis of indoles in the plasma of healthy women of reproductive age (n = 25) as controls and patients with polycystic ovarian syndrome (n = 61). The results showed that the concentrations of indoles in the plasma were significantly different between the two groups, and IND was found to be a risk factor and a potential diagnostic biomarker for polycystic ovarian syndrome (PCOS). The method is simple, sensitive and suitable for use in clinical testing and laboratory research.
Sometimes chemists are able to propose two or more mechanisms that are consistent with the available data. If a proposed mechanism predicts the wrong experimental rate law, however, the mechanism must be incorrect.Welcome to check out more blogs about 481-72-1, in my other articles. Quality Control of Aloe-emodine.
Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles