Development of a new chromatographic method for estimation of Dolasetron in bulk and pharmaceutical dosage form by RP-HPLC was written by Raju, B.;Silas, P.. And the article was included in World Journal of Pharmaceutical Research in 2017.Synthetic Route of C19H20N2O3 This article mentions the following:
Materials and Methods: HPLC of Waters (Model: Alliance 2695) with Phenomenex Luna C18 (4.6 mm I.D. × 250 mm, 5μm) column was used for chromatog. separation It contains waters injector and PDA Detector (Deuterium). Mobile phase consists of Acetonitrile: Water (50:50% volume/volume) and flow rate adjusted was 1ml/min. Wavelength selected for detection was 285nm and injection volume was 10μl. Results and discussion: By using the developed method, retention time of Dolasetron was found to be 3.008 resp. The method has been validated for linearity, accuracy and precision. Linearity of Dolasetron were in the range 10-50μg/mL resp. The percentage recoveries obtained for Dolasetron were found to be in range of 99.6 LOD and LOQ were found to be 1.16μg/mL and 3.5μg/mL for Dolasetron. Conclusion: A rapid and precise Reverse Phase High Performance Liquid Chromatog. method has been developed for the validated of Dolasetron, in its pure form as well as in tablet dosage form. Chromatog. was carried out on a Symmetry C18 (4.6 × 250mm, 5μm) column using a mixture of Acetonitrile and Water as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 285nm. The retention time of the Dolasetron was 3.0μ 0.02min. The method produce linear responses in the concentration range of 10-50ppm of Dolasetron. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. In the experiment, the researchers used many compounds, for example, rel-(5s,6R,8r,9aS)-3-Oxooctahydro-1H-2,6-methanoquinolizin-8-yl 1H-indole-3-carboxylate (cas: 115956-12-2Synthetic Route of C19H20N2O3).
rel-(5s,6R,8r,9aS)-3-Oxooctahydro-1H-2,6-methanoquinolizin-8-yl 1H-indole-3-carboxylate (cas: 115956-12-2) belongs to indole derivatives. The indole subunit is an almost ubiquitous component of biologically active natural products, and its study has been the focus of research for decades. They are capable of binding to a variety of receptors with high affinity and thus have applications in a wide range of therapeutic areas.Synthetic Route of C19H20N2O3
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles