Synthesis and characterization of new isatin derivatives for cytototoxic activity was written by Shoba, Rani B.;Blessi, Priyanka K.;Gade, Sammaiah. And the article was included in International Journal of Pharmacy and Biological Sciences in 2015.Synthetic Route of C8H4N2O4 This article mentions the following:
A series of N-(6-benzoyl-1H-benzo[d]imidazol-2-yl)-2-(2-oxoindolin-3-ylidene) hydrazine carboxamides were synthesized by treating (5-benzoyl-1H-benzo[d]imidazol-2-yl) carbamate with different substituted isatins. The novel compounds were characterized on the basis of spectral (FT-IR, 1H NMR, Mass) anal. All the synthesized derivatives were screened for cytotoxic activity against Hela cancer cell lines using MTT assay. All the synthesized compounds produced a dose dependent inhibition of growth of the cells. The IC50 values of all the synthetic test compounds were found between 17.32-55.57. The potency of (IC50 values) of cytotoxic activity of compounds was compared with that of known cytotoxic agent, Cisplatin. Almost all the synthesized novel compounds showed the most potent activity against all cell lines. These results indicate that C-5 substituted derivatives may be useful leads for anticancer drug development in future. In the experiment, the researchers used many compounds, for example, 7-Nitroindoline-2,3-dione (cas: 112656-95-8Synthetic Route of C8H4N2O4).
7-Nitroindoline-2,3-dione (cas: 112656-95-8) belongs to indole derivatives. Indole produced by Proteus, Pseudomonas, Escherichia and other species was shown to be a growth promoting factor in Arabidopsis thaliana. It is used in perfumery and in making tryptophan, an essential amino acid, and indoleacetic acid (heteroauxin), a hormone that promotes the development of roots in plant cuttings.Synthetic Route of C8H4N2O4
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles