Chemistry is the experimental and theoretical study of materials on their properties at both the macroscopic and microscopic levels.In a patent， name: 4-Chloro-1H-indole-2-carboxylic acid, Which mentioned a new discovery about 24621-73-6
The beta-catenin/T-cell factor (Tcf) protein-protein interaction (PPI) plays a critical role in the beta-catenin signaling pathway which is hyperactivated in many cancers and fibroses. Based on compound 1, which was designed to target the Tcf4 G13ANDE17 binding site of beta-catenin, extensive structure-activity relationship studies have been conducted. As a result, compounds 53 and 57 were found to disrupt the beta-catenin/Tcf PPI with the Ki values of 0.64 and 0.44 muM, respectively, and exhibit good selectivity for beta-catenin/Tcf over beta-catenin/E-cadherin and beta-catenin/adenomatous polyposis coli (APC) PPIs. The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assays revealed that 56, the ethyl ester of 53, was more potent than 53 in inhibiting viability of most of the Wnt/beta-catenin hyperactive cancer cells. Further cell-based studies indicated that 56 disrupted the beta-catenin/Tcf PPI without affecting the beta-catenin/E-cadherin and beta-catenin/APC PPIs, suppressed transactivation of Wnt/beta-catenin signaling in dose-dependent manners, and inhibited migration and invasiveness of Wnt/beta-catenin-dependent cancer cells.
I hope this article can help some friends in scientific research. I am very proud of our efforts over the past few months and hope to 24621-73-6, help many people in the next few years.HPLC of Formula: C9H6ClNO2
Reference：
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles