A catalyst don’t appear in the overall stoichiometry of the reaction it catalyzes, SDS of cas: 3131-52-0, but it must appear in at least one of the elementary reactions in the mechanism for the catalyzed reaction. 3131-52-0, Name is 5,6-Dihydroxyindole, molecular formula is C8H7NO2. In a Article, authors is Anjugam, Mahalingam£¬once mentioned of 3131-52-0
A study on beta-glucan binding protein (beta-GBP) and its involvement in phenoloxidase cascade in Indian white shrimp Fenneropenaeus indicus
The present study reports the purification of novel immune molecule beta-1, 3 glucan binding protein from the heamolymph of the Indian white shrimp, Fenneropenaeus indicus (Fibeta-GBP). The purified Fibeta-GBP had 95 kDa molecular weight in SDS-PAGE analysis. MALDI-TOF/TOF analysis revealed that the purified Fibeta-GBP showed similarity to various crustacean proteins; 48 and 46% similarity was observed for beta-1, 3 glucan binding protein of Chinese white shrimp Fenneropenaeus chinensis and banana shrimp Fenneropenaeus merguiensis, with MOWSE score of 3.11e + 12 and 2.05e + 8, respectively. The phenoloxidase activity (PO) of Fibeta-GBP was evaluated and, in the presence of laminarin, PO activity increased significantly. Substrate specificity assay demonstrated that Fibeta-GBP had the specific binding site for soluble or insoluble beta-glucan (laminarin), since the PO activity increased in the presence of laminarin when compared to other sugars. Enzymatic activities revealed that the optimum temperature and pH for Fibeta-GBP activating PO were 40 C and pH 7?8. Moreover, even at 100 C Fibeta-GBP enhanced PO activity highlighting that Fibeta-GBP was thermostable and thermophilic in nature. Among various divalent metallic ions, Fibeta-GBP significantly promoted the PO activity in presence of Mg2+ and Ca2+. The breakdown of para nitroanilide from Nalpha?Benzoyl-L-Arginine 4-Nitroanilide hydrochloride showed that serine protease activity was induced by Fibeta-GBP and also increased concentration of Fibeta-GBP evoked the activity. Furthermore, hemolytic activity tests revealed that PO reaction product induced RBC membrane damage and cell shrinkage. Lastly, Baclight bacterial viability assays showed maximum killing effect of PO reaction product on both Gram positive and Gram negative bacteria.
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Reference£º
Indole alkaloid derivatives as building blocks of natural products from?Bacillus thuringiensis?and?Bacillus velezensis?and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles