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Metabolomics Analysis Reveals Major Differential Metabolites and Metabolic Alterations in Tea Plant Leaves (Camellia sinensis L.) Under Different Fluorine Conditions
Tea plant (Camellia sinensis L.) is capable of accumulating a large amount of fluorine (F) in leaves without showing toxicity symptoms and thus offers a good model for exploring F tolerance mechanisms. Here, gas chromatography time-of-flight mass spectrometry (GC-TOF?MS) was used to investigate metabolic changes in leaves of tea seedlings under control (0?mM), low F (0.2?mM) and high F (0.8?mM) conditions. Differentially changed metabolites such as galacturonic acid, lactose, fructose, malic acid, alanine were identified by the comparison among the three F treatment groups. A pathway map depicted based on the KEGG database reflected the involvement of pectin biosynthesis metabolism in F stress response. The gene expression and enzyme activity of key enzymes involved in pectin biosynthesis pathway and the content of pectic polysaccharides were increased by exogenous F treatments, indicating the promotion effect of F on the pectin biosynthesis. Pectin was also immunochemically stained in vivo using monoclonal antibody (2F4), which confirmed the increment. The increased pectin might contribute to combining the exogenous F in tea leaves. This research provided some novel insights into further research on F detoxification of plants.
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Indole alkaloid derivatives as building blocks of natural products from?Bacillus thuringiensis?and?Bacillus velezensis?and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles