Wang, Xiaodong; Fan, Li; Wang, Yubin; Zhang, Caihong; Liang, Wenting; Shuang, Shaomin; Dong, Chuan published the artcile< Visual monitoring of the lysosomal pH changes during autophagy with a red-emission fluorescent probe>, Quality Control of 950846-89-6, the main research area is lysosome pH autophagy red emission fluorescent probe.
Autophagy plays crucial roles in maintaining normal intracellular homeostasis. Mol. probes capable of monitoring lysosomal pH changes during autophagy are still highly required yet challenging to develop. Here, a lysosome-targeting fluorescent pH probe, RML (I), is presented by introducing a methylcarbitol unit as the lysosome-targeting group to rhodamine B, which is highly sensitive to pH changes. RML exhibits remarkable pH-dependent behavior at 583 nm with a fluorescent enhancement of >148-fold. The pKa value is determined as 4.96, and the linear response with pH changes from 4.50-5.70, which is favorable for lysosomal pH imaging. Also RML diffuses selectively into lysosomes using confocal fluorescence microscopy. Using RML, the authors have successfully visualized autophagy by monitoring the lysosomal pH changes.
Journal of Materials Chemistry B: Materials for Biology and Medicine published new progress about Autophagy. 950846-89-6 belongs to class indole-building-block, and the molecular formula is C30H36N4O2, Quality Control of 950846-89-6.
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Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles