HPLC of Formula: C9H10O3. Authors Yadav, U; Vanjari, Y; Laxmikeshav, K; Tokala, R; Niggula, PK; Kumar, M; Talla, V; Kamal, A; Shankaraiah, N in BENTHAM SCIENCE PUBL LTD published article about in [Yadav, Upasana; Vanjari, Yogesh; Laxmikeshav, Kritika; Tokala, Ramya; Niggula, Praveen K.; Shankaraiah, Nagula] Natl Inst Pharmaceut Educ & Res NIPER, Dept Med Chem, Hyderabad 500037, India; [Kumar, Manoj; Talla, Venu] Natl Inst Pharmaceut Educ & Res NIPER, Dept Pharmacol & Toxicol, Hyderabad 500037, India; [Kamal, Ahmed] Jamia Hamdard, Sch Pharmaceut Educ & Res SPER, New Delhi 110062, India in 2021, Cited 66. The Name is 3,4-Dimethoxybenzaldehyde. Through research, I have a further understanding and discovery of 120-14-9
Objective: To synthesize a series of phenanthrene-thiazolidinedione hybrids and explore their cytotoxic potential against human cancer cell lines of A-549 (lung cancer), HCT-116 and HT-29 (colon cancer), MDA MB-231 (triple-negative breast cancer), BT-474 (breast cancer) and (mouse melanoma) B16F10 cells. Methods: A new series of phenanthrene-thiazolidinedione hybrids was synthesized via Knoevenagel condensation of phenanthrene-9-carbaldehyde and N-alkylated thiazolidinediones. The cytotoxicity (IC50) of the synthesized compounds was determined by MTT assay. Apoptotic assays like (AO/EB) and DAPI staining, cell cycle analysis, JC-1 staining and Annexin V binding assay studies were performed for the most active compound (Z)- 3-(4-bromobenzyl)-5-((2,3,6,7-tetramethoxyphenanthren-9-yl)methylene)thiazolidine-2,4-dione (17b). Molecular docking, dynamics and evaluation of pharmacokinetic (ADME/T) properties were also carried out by using Schrodinger. Results and Discussion: From the series of tested compounds, 17b unveiled promising cytotoxic action with an IC50 value of 0.985 +/- 0.02 mu M on HCT-116 human colon cancer cells. The treatment of HCT-116 cells with 17b demonstrated distinctive apoptotic morphology like shrinkage of cells, horseshoe-shaped nuclei formation and chromatin condensation. The flow-cytometry analysis revealed the G(0)/G(1) phase cell cycle arrest in a dosedependent fashion. The AO/EB, DAPI, DCFDA, Annexin-V and JC-1 staining studies were performed in order to determine the effect of the compound on cell viability. Computational studies were performed by using Schrodinger to determine the stability of the ligand with the DNA. Conclusion: The current study provides an insight into developing a series of phenanthrene thiazolidinedione derivatives as potential DNA interactive agents which might aid in colon cancer therapy.
HPLC of Formula: C9H10O3. About 3,4-Dimethoxybenzaldehyde, If you have any questions, you can contact Yadav, U; Vanjari, Y; Laxmikeshav, K; Tokala, R; Niggula, PK; Kumar, M; Talla, V; Kamal, A; Shankaraiah, N or concate me.
Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles