Wu, Xiuli published the artcileValidated LC method for the enantiomeric separation of EAI045 on chiral stationary phase, Product Details of C19H14FN3O3S, the publication is Journal of Chromatographic Science (2020), 58(6), 562-568, database is CAplus and MEDLINE.
A simple and accurate chiral liquid chromatog. method was developed for enantiomeric resolution and determination of 2-(5-fluoro-2-hydroxyphenyl)-2-(1-oxo-2,3-dihydro-1H-isoindol-2-yl)-N-(1,3-thiazol-2-yl)acetamide (EAI045). The enantiomers of EAI045 were baseline resolved on a Chiralpak AD-H (250 mm x 4.6 mm, 5μm) column using a mobile phase system containing n-hexane: 2-propanol (75: 25 volume/volume) at a flow rate of 1 mL min-1 at 30°C. The eluted analytes were subsequently detected with an UV detector at 254 nm. The effects of organic modifiers and temperature on the enantioselectivity and resolution of the enantiomers were evaluated. The calibration curves were plotted within the concentration range between 2 and 600μg mL-1 (n = 11), and recoveries between 98.74% and 101.52% were obtained, with relative standard deviation < 1.4%. The limit of detection and limit of quantitation for R-enantiomer were 0.94 and 3.07μg mL-1 and for S-enantiomer were 0.86 and 2.84μg mL-1, resp. The validated method was found to be suitable for enantiomeric separation and sufficiently accurate for the determination of enantiomeric purity of EAI045 in bulk drugs.
Journal of Chromatographic Science published new progress about 1942114-09-1. 1942114-09-1 belongs to indole-building-block, auxiliary class Indoline,Thiazole,Fluoride,Amine,Benzene,Amide,Alcohol,Protein Tyrosine Kinase/RTK, name is 2-(5-Fluoro-2-hydroxyphenyl)-2-(1-oxoisoindolin-2-yl)-N-(thiazol-2-yl)acetamide, and the molecular formula is C11H10ClNO, Product Details of C19H14FN3O3S.
Referemce:
https://www.nature.com/articles/s41429-020-0333-2,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles