Yeyeodu, Susan T. published the artcileA rapid, inexpensive high throughput screen method for neurite outgrowth, SDS of cas: 330161-87-0, the publication is Current Chemical Genomics (2010), 74-83, database is CAplus and MEDLINE.
Neurite outgrowth assays are the most common phenotypic screen to assess chem. effects on neuronal cells. Current automated assays involve expensive equipment, lengthy sample preparation and handling, costly reagents and slow rates of data acquisition and anal. We have developed a high throughput screen (HTS) for neurite outgrowth using a robust neuronal cell model coupled to fast and inexpensive visualization methods, reduced data volume and rapid data anal. Neuroscreen-1 (NS-1) cell, a subclone of PC12, possessing rapid growth and enhanced sensitivity to NGF was used as a model neuron. This method reduces preparation time by using cells expressing GFP or native cells stained with HCS CellMask Red in a multiplexed 30 min fixation and staining step. A 2 × 2 camera binning process reduced both image data files and anal. times by 75% and 60% resp., compared to current protocols. In addition, eliminating autofocus steps during montage generation reduced data collection time. Pharmacol. profiles for stimulation and inhibition of neurite outgrowth by NGF and SU6656 were comparable to current standard method utilizing immunofluorescence detection of tubulin. Potentiation of NGF-induced neurite outgrowth by members of a 1,120-member Prestwick compound library as assayed using this method identified six mols., including etoposide, isoflupredone acetate, fludrocortisone acetate, thioguanosine, oxyphenbutazone and gibberellic acid, that more than doubled the neurite mass primed by 2 ng/mL NGF. This simple procedure represents an important routine approach in high throughput screening of large chem. libraries using the neurite outgrowth phenotype as a measure of the effects of chem. mols. on neuronal cells.
Current Chemical Genomics published new progress about 330161-87-0. 330161-87-0 belongs to indole-building-block, auxiliary class Protein Tyrosine Kinase/RTK,Src, name is SU6656, and the molecular formula is C10H18BNO2, SDS of cas: 330161-87-0.
Referemce:
https://www.nature.com/articles/s41429-020-0333-2,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles