On March 31, 2022, Zhang, Hongjun; Cheng, Zeguang; Wei, Litian; Yu, Xinjun; Wang, Zhao; Zhang, Yinjun published an article.Formula: C9H9NO2 The title of the article was Semi-rational protein engineering of a novel esterase from Bacillus aryabhattai (BaCE) for resolution of (R,S)-ethyl indoline-2-carboxylate to prepare (S)-indoline-2-carboxylic acid. And the article contained the following:
A gene encoding an esterase from Bacillus aryabhattai (BaCE) was identified, synthesized and efficiently expressed in the Escherichia coli system. A semi-rational protein engineering was applied to further improve the enzymes enantioselectivity. Under the guidance of the mol. docking result, a single mutant BaCE-L86Q and a double mutant BaCE-L86Q/G284E were obtained, with its Emax value 6.4 times and 13.9 times of the wild-type BaCE, resp. The recombinant BaCEs were purified and characterized. The overwhelming E value demonstrated that BaCE-L86Q/G284E was a promising biocatalyst for the biol. resolution to prepare (S)-indoline-2-carboxylic acid. The experimental process involved the reaction of H-Idc-OH(cas: 79815-20-6).Formula: C9H9NO2
The Article related to bacillus escherichia esterase protein engineering enantioselectivity, (s)-indoline-2-carboxylic acid, biocatalysis, enantioselective, esterase, overexpression, protein engineering and other aspects.Formula: C9H9NO2
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles