On October 31, 2019, Zhang, Yin-Jun; Chen, Chang-Sheng; Liu, Hao-Tian; Chen, Jia-Lin; Xia, Ying; Wu, Shi-Jin published an article.Computed Properties of 79815-20-6 The title of the article was Purification, identification and characterization of an esterase with high enantioselectivity to (S)-ethyl indoline-2-carboxylate. And the article contained the following:
Objective: To purify an esterase which can selectively hydrolyze (R,S)-Et indoline-2-carboxylate to produce (S)-indoline-2-carboxylic acid and characterize its enzymic properties. Results: An intracellular esterase from Bacillus aryabhattai B8W22 was isolated and the purified protein was identified as a carboxylesterase by MALDI-TOF mass spectrometry. The enzyme (named BaCE) was 59.03-fold purification determined to be of approx. 35 kDa. Its specific activity was 0.574 U/mL with 20% yield. The enzyme showed maximum activity at pH 8.5 and 30°C and was stable at 20-30°C using pNPB as the substrate. The esterase demonstrated high enantioselectivity toward (S)-Et indoline-2-carboxylate with 96.55% e.e.p at 44.39% conversion, corresponding to an E value of 133.45. Conclusions: In this study, a new esterase BaCE with an apparent mol. mass of 35 kDa was purified to homogeneity for the first time. The esterase from Bacillus aryabhattai B8W22 was isolated with a purification more than 59-fold and a yield of 20% by anion exchange chromatog. and hydrophobic interaction chromatog. And its biochem. characterization were described in detail with pNPB as substrate. It displayed high enantioselectivity toward (S)-Et indoline-2-carboxylate. We next plan to highly express esterase BaCE in Escherichia coli, and apply it to industrial production of (S)-indoline-2-carboxylic acid. The experimental process involved the reaction of H-Idc-OH(cas: 79815-20-6).Computed Properties of 79815-20-6
The Article related to esterase enantioselectivity ethyl indoline 2carboxylate, (s)-indoline-2-carboxylic acid, bacillus aryabhattai b8w22, characterization, esterase, purification, Enzymes: Separation-Purification-General Characterization and other aspects.Computed Properties of 79815-20-6
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Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles