Synthesis of a new near-IR fluorescent reagent and study on its in-situ dimer’s interaction with DNA was written by Zheng, Hong;Li, Dong-Hui;Wu, Min;Chen, Qiu-Ying;Xu, Jin-Gou. And the article was included in Gaodeng Xuexiao Huaxue Xuebao in 1999.Reference of 146368-07-2 This article mentions the following:
A new anionic Near-IR fluorescent cyanine dye was synthesized and its absorption and fluorescence spectra in water and in the solution of cationic surfactant-hexadecyltrimethyl-ammonium bromide (CTAB) were studied. The results showed that the maximum excitation wavelength and emission wavelength were 765 nm and 795 nm in water, resp. In the presence of low concentration of CTAB, the dye formed ionic associate with CTAB was made higher to form the pre-micellar aggregation, the fluorescence intensity decreased dramatically because of forming a low-fluorescent in-situ dimeric dye. When the concentration of CTAB was above the critical micelle concentration (cmc), the dimeric dye dissociated, and the fluorescence intensity of Near-IR cyanine was restored, and the maximum emission wavelength was bathochromated from 795 nm to 812 nm. The possibility of using low-fluorescent in-situ dimeric dye as a Near-IR region fluorescent probe for DNA was also studied. In the experiment, the researchers used many compounds, for example, 1-Ethyl-2,3,3-trimethyl-3H-indol-1-ium-5-sulfonate (cas: 146368-07-2Reference of 146368-07-2).
1-Ethyl-2,3,3-trimethyl-3H-indol-1-ium-5-sulfonate (cas: 146368-07-2) belongs to indole derivatives. Indole, also called Benzopyrrole, a heterocyclic organic compound occurring in some flower oils, such as jasmine and orange blossom, in coal tar, and in fecal matter. It is used in perfumery and in making tryptophan, an essential amino acid, and indoleacetic acid (heteroauxin), a hormone that promotes the development of roots in plant cuttings.Reference of 146368-07-2
Referemce:
Indole alkaloid derivatives as building blocks of natural products from聽Bacillus thuringiensis聽and聽Bacillus velezensis聽and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles